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HostDetect CHO PCR DNA Quant Kit
HostDetect CHO PCR DNA Quant Kit
HostDetect PCR DNA Quant Kit
| Feature | Specification |
|---|---|
| Analytical Sensitivity | ≥ 0.01 pg/rxn |
| Linear Concentration Range | R2 ≥ 0.98, Eff%= 100±20% |
| Sizing Precision RSD (CV) | CV < 5% at 0.01 pg/rxn |
Product variant
Unit Size: 192 rxns
Part #:
DXMDX-RGT-1003
For research use only. Not for use in diagnostic procedures.
HostDetect CHO PCR DNA Quant Kit
HostDetect PCR DNA Quant Kit
HostDetect CHO PCR DNA Quant Kit
Product information
Overview
The HostDetect CHO PCR DNA Quant Kit utilizes sequence-specific primers and TaqMan® probe to amplify the Alu-equivalent gene of CHO genomic DNA for residual host genomic identification. A primer/probe set to detect Internal Control (IC), is also included for monitoring entire process from extraction to real-time PCR. The reagents also use a dUTP/UNG carryover prevention system to avoid contamination of PCR products.
This kit is specific for DNA from the CHO genome and cannot detect human or environmental DNA that might be introduced during sample handling.
Key Highlights:
- Quantify CHO genomic DNA with as little as 0.01 pg per PCR reaction in < 2 hours of PCR
- Internal control detection for process monitoring from sample extraction to PCR
- High specificity: No cross-reactivity with unrelated DNA
- Flexible extracted DNA input volume up to 10 µL per well
- Scalable reaction numbers from 1 to up to 192 per kit
Additional product information
HostDetect™ CHO PCR DNA Quant Kit Workflow
The probes for the CHO genomic DNA and Internal Control detection are labeled with FAM and HEX/VIC fluorescent dyes, respectively, to generate target-specific signal. ROX is used for Passive Reference. Any Real-time qPCR Instruments with FAM™ and VIC®/HEX™ channels can be used with this assay.
HostDetect™ CHO PCR DNA Quant Kit Analytical Range**
**: ATCC or USP reference standards for residual DNAs are not included in the kit. Customer must order these separately from ATCC or USP web sites.
Specifications
| Analytical Sensitivity |
≥ 0.01 pg/rxn
|
|---|---|
| Limit of Quantification |
100 pg to 0.01 pg/rxn
|
| Linear Concentration Range |
R2 ≥ 0.98, Eff%= 100±20%
|
| Sizing Precision RSD (CV) |
CV < 5% at 0.01 pg/rxn
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-25C to -15C
|
| Unit Size |
192 rxns
|
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