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  • Cellometer Spectrumイメージサイトメトリーシステム

Cellometer Spectrumイメージサイトメトリーシステム

Cellometer Spectrumイメージサイトメーターは、蛍光試薬やキットを用いた細胞ベースアッセイに対応した、高性能でモジュール式の細胞カウンターです。目的のアッセイや試薬に合わせてカスタマイズできます。

研究用です。診断用にはご使用いただけません。
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Cellometer Spectrum Image Cytometry System
Cellometer Spectrumイメージサイトメトリーシステム
Cellometer Spectrum Image Cytometry System
Cellometer Spectrum Image Cytometry System
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Product information

  • Overview
  • Additional product information
  • Specifications

Overview

Cellometer Spectrumイメージサイトメーターは、あらかじめ設計されたテンプレートを使用し、フローサイトメトリーに類似したデータを取得できる、カスタマイズ可能な細胞計数・サイトメトリー装置です。

FCS expressを使用して、細胞集団データをヒストグラム、散布図、ドットプロット、または等高線プロットとして迅速に可視化できます。フィルタセットはユーザーが変更可能で、目的のアッセイや試薬に合わせて装置をカスタマイズできます。

様々な細胞解析ソリューション

基本的なセルカウンティング、初代細胞の生存率評価、細胞ベースアッセイに対応

  • PBMCs
  • 幹細胞
  • 脂肪細胞
  • 神経細胞
  • 肝細胞
  • 樹状細胞
  • 上皮細胞
  • ケラチノサイト
  • リンパ球
  • 脾細胞
  • 単球
  • 酵母
  • 白血球
  • 培養細胞
  • 核を持つ細胞

蛍光二重染色による、初代細胞の正確な生存率評価

赤血球の影響を受けません。骨髄、末梢血、臍帯血を溶血処理なしで解析可能

ユーザー変更可能な蛍光フィルター

6種類のカラーオプションから選択し、二重蛍光アッセイを実施可能

高度な細胞解析のための独自アルゴリズムを搭載

肝細胞、脂肪細胞など、さまざまな細胞タイプの濃度と生存率を測定可能

迅速な結果出力

細胞画像の取得、細胞数・サイズ測定、生存率の算出を、1サンプルあたり30秒以内で実施

シンプルな細胞ベースアッセイに対応

  • 20μLの少量サンプルで測定可能
  • 細胞数、濃度、生存率を測定可能
  • 二重抗体アッセイに対応
  • GFP/RFP導入細胞の解析に対応
  • セルヘルスおよび細胞ベースアッセイに対応:
    • アポトーシス
    • 細胞増殖
    • 細胞周期
    • ミトコンドリア膜電位
    • 貪食能
    • 表面マーカー解析

Additional product information

Advantages of Cellometer Spectrum

View Cell Images

  • Visually check cell morphology
  • Ensure only cells of interest are counted
  • Archive and re-analyze cell images
  • Export images for publication
     

Proprietary Pattern-Recognition Software

  • Count individual cells in clusters
  • Count irregular-shaped cells
  • Count cells based on size
  • Eliminate debris from cell counts
     

Non-Fluidic Platform

  • Disposable counting chambers – no washing
  • Compatible with fragile cells
  • Maintenance-free
  • Robust optics modules and LED light sources
     

IQ/OQ Validation and GMP/GLP Accessories

  • Installation Qualification reagents/protocol
  • Operational Qualification reagents/protocol
  • On-site IQ and OQ performance
  • GMP/GLP Software Module


Fluorescent Cell Counting

Brightfield and AO cell image
  • Automatic identification of Acridine Orange (AO) stained cells (shown above) and Propidium Iodine (PI) stained cells (shown below).
  • Image and count up to 2×107cells/mL
Brightfield and PI cell image


User-Changeable Fluorescence Optics Modules*

The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.

Optics Module Fluorophores Nucleic Acid Stains Fluorescent Proteins and other Fluorescent Cell Reagents
S1-452-365

Ex. 370 nm (BW: 36 nm)

Em: 452 nm (BW: 45 nm)
BV421

V450

Pacific Blue
Hoechst 33342

DAPI

ViaStain™ Dead Cell Nuclear Blue
Calcein AM Violet

CTV (CellTrace Violet)

Tracer Blue

BFP
S1-534-470

Ex: 475 nm (BW: 42 nm)

Em: 534 nm (BW:42 nm)
FITC

AlexaFluor® 488
AO (acridine orange)

SYTO®9, SYTO®13

SYTOX®Green

SYTO®BC
GFP

YFP

Calcein AM

CFSE

JC-1
S1-594-470

Ex. 475 nm (BW: 42 nm)

Em: 594 nm (LP - Long Pass)
    Chlorophyll A

Chlorophyll B
S1-605-527

Ex: 525 nm (BW: 45 nm)

Em: 605 nm (BW: 64 nm)
AlexaFluor® 546

AlexaFluor® 555

Cy3®

PE (R-phycoerythrin)

RFP
PI (propidium iodide)

EB (ethidium bromide)

SYTOX® Orange
RFP

mCherry

TdTomato

TurboRed

TMRE/TMRM

JC-1
S1-655-527

Ex: 525 nm (BW: 45 nm)

Em: 655 nm (BW: 40 nm)
  PI (propidium iodide)

EB (ethidium bromide)

7-AAD
Nile Red
S1-692-620

Ex: 628 nm (BW: 40 nm)

Em: 692 nm (BW: 40 nm)
AlexaFluor® 647

APC (allophycocyanin)

Cy5®
SYTOX® Red iRFP670

CellTrace Far Red

Cell Tracker Deep Red

LysoTracker Deep Red

 

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Revvity technical support regarding compatibility of other reagents. Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
 

Cellometer Spectrum Specifications 

Includes
  • Cellometer Spectrum image cytometer
  • Cellometer Software
  • FCS Express 6 analysis software (2 licenses)
  • Two Fluorescent Optics Modules
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
Magnification selection
  • 10x (ex. yeast, platelets, algae)
Instrument specifications
  • Weight: 24.0 lbs. (10.9 kg)
  • Dimensions: Width: 6.0" (15.2 cm), Depth: 8.5" (21.6 cm), Height: 14.0" (35.6cm)
  • Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to Instrument: 12 VDC, 3.34A
Available accessories
  • PC / Laptop
  • Minimum Requirements (If purchasing Cellometer without PC laptop)
Imaging performance
  • 10x Magnification:
    • Cell Size: 0.5 - 25 microns
    • Concentration Range: 105 - 107 cells/ml
  • Brightfield imaging, fluorescent imaging and pattern-recognition software to quickly decluster, identify and count individual cells.

Specifications

Dimensions 6.0 in (W) x 8.5 in (D) x 14.0 in (H)
Weight
10.9 kg
Brand
Revvity
Imaging Modality
蛍光・明視野
Model
Cellometer Spectrumイメージサイトメトリーシステム
Technology
Cellometer Software
Unit Size
1 each

Citations

Resources

Are you looking for resources, click on the resource type to explore further.

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  • Application Note:-:2
  • Brochure:-:2
  • Datasheet:-:1
  • Flyer:-:4
  • Literature - Publication Review:-:3
  • Specification Sheet:-:1
  • Whitepaper:-:6
  • eBook:-:2
13-18 of 21 Resources
Whitepaper Icon
Whitepaper
Practical cell counting method selection to increase the quality of results based on ISO cell counting standards

The importance of cell counting has increased significantly in the last decade due to the major advances in the fields of cell and...

Learn more
Publication Icon
Literature - Publication Review
Publication list for Cellometer, Cellaca and Celigo cell counting and image cytometry systems
Learn more
Brochure Icon
Brochure
Solutions for infectious disease research and discovery

Truly understanding infectious diseases is the first step toward containment and eradication. From biology to breakthrough, our...

Learn more
eBook Icon
eBook
Solutions for precision medicine research

The need for better profiling is clear, and technological advances are making it possible to optimize clinical trials and...

Learn more
Flyer Icon
Flyer
Sources of variation in cell counting and how to avoid them
Learn more
Specification Sheet Icon
Specification Sheet
Specification sheet for the Cellometer Spectrum image cytometer.
Learn more
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