Cellometer Ascend 自動セルカウンター

The loading volume of stained sample in ASD-CHM3 slides is 20 µl. ASD-CHM8 slides require 10 µl of sample. Typically, you will stain your cell sample 1:1 with AOPI dual-fluorescent viability dye, so you can perform a cell count with as little as 5 µl of cell sample.

Yes, load the counting chamber with as many or as few samples as you want to count. The Cellometer Ascend software will allow you to indicate which chambers contain samples you wish to count and the rest of the slide can be saved for later use.

The Cellometer Ascend is designed to identify live and dead nucleated cells, even in very messy samples with RBCs and debris or in irregular-shaped cell populations. By using the AOPI dual-fluorescence viability dye, only nucleated cells are counted and RBCs and debris are ignored (this method is so specific, it even works in whole blood without lysing!). The Cellometer Ascend will show you the images it captured and which cells were counted so that you can visually verify the results and trust your data.

Yes, the Cellometer Ascend is designed to count isolated nuclei and comes with a pre-set protocol for nuclei counting which was created based on samples prepared for single cell sequencing.

The Cellometer Ascend uses autofocus for ease of use, speed, and user-to-user consistency. The autofocus can be configured to focus based on the cells in your sample, or based on the slide position. Slide-based focus enables consistent focus in the correct location, even if your cell sample concentration is very low.