ATPlite 1step 3D, 1,000 Assay Points

ATP serves as a reliable indicator of cell viability because it is found in all metabolically active cells. As cells undergo necrosis or apoptosis, ATP levels decrease rapidly, making ATP measurement an effective way to assess cytotoxic, antiproliferative, and cell growth–related effects.

The ATPlite 3D and ATPlite 1step 3D luminescence assays use proprietary technology to evaluate cell proliferation and cytotoxicity in 3D cultured mammalian cells. These assays detect light generated from the reaction between ATP and the added enzymes luciferase and D-luciferin.

The Luminescence ATP 1step 3D Assays are based on firefly (Photinus pyralis) luciferase and provide a convenient method for monitoring ATP levels. This assay serves as an alternative to colorimetric, fluorometric, high-content imaging, and radioisotopic approaches for quantifying proliferation and cytotoxicity in mammalian cells grown as 3D spheroids. ATP measurement can be applied to evaluate the cytocidal, cytostatic, and proliferative effects of various drugs, biological response modifiers, and other bioactive compounds.

The ATPlite 1step, 3D 100 mL kit accommodates 1,000 assays in a 96-well plate using 100 µL per well and 4,000 assays in a 384-well plate using 25 µL per well.

Kit components:

• 10 vial of Lyophilized Substrate Solution
• 1 x 100 mL of Substrate Buffer Solution
• 2 vial of Lyophilized ATP Standard Solution

Storage: Buffer and vials should be stored at 4°C.

Features and benefits:

• Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after the last pipetting step
• Rapid: results generated in 25 - 30 minutes
• Simple and reproducible: only one reagent addition step, no separation steps. A final transfer step may be necessary, depending on the type of plate used for Spheroid growth.
• Homogeneous assay: no cell harvesting or centrifugation required
• Wide linear dynamic range: detect ATP from spheroids made by seeding as few as 200 cells, and up to 5000 cells
• Convenient storage conditions (2 - 8 °C)
• No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards, unpleasant odors and the need for hood work
• Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values