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ATP Standard
ATP Standard
ATP standard primary image
Lyophilized Adenosine 5'-Triphosphate (ATP) disodium salt is specifically formulated for the generation of accurate standard curves in bioluminescence-based detection assays. Upon reconstitution with ultrapure, nuclease-free water it provides a reliable reference standard for quantifying ATP concentrations using the firefly luciferase reaction.
| Feature | Specification |
|---|---|
| Application | 細胞生存率 |
Lyophilized Adenosine 5'-Triphosphate (ATP) disodium salt is specifically formulated for the generation of accurate standard curves in bioluminescence-based detection assays. Upon reconstitution with ultrapure, nuclease-free water it provides a reliable reference standard for quantifying ATP concentrations using the firefly luciferase reaction.
Product variant
For research use only. Not for use in therapeutic or diagnostic procedures.
ATP Standard
ATP standard primary image
ATP Standard
Product information
Overview
Our lyophilized ATP Standard is a reagent designed for calibrating luminescence assays with precision. Each vial undergoes testing to ensure low background interference and optimal reactivity with luciferase-luciferin reagents. The lyophilized powder offers a longer shelf-life and heat resistance compared to liquid formats. Ideal for constructing multi-point standard curves, this product enables researchers to convert luminescence units into absolute molar concentrations with confidence and reproducibility.
Preparation of 10 mM ATP Solution:
- Check the ATP amount on the vial label. (provided in μmole)
- Calculate the volume of water needed using the formula: Volume of water (μL) = ATP amount (μmole) × 100
- Example:If the label says 11.7 μmole, then: Volume = 11.7 × 100 = 1170 μL
- Add the calculated volume of water* to the vial.
- Dissolve the ATP completely by swirling the vial gently for at least one minute.
This results in a 10mM ATP solution in water.
Note: Ensure the ATP is fully dissolved before use.
*Use deionized, distilled or water for injection grade water.
Specifications
| Application |
Cell Viability
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
ATPlite
|
| Cellular or Signaling Pathway |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
| Detection Modality |
Luminescence
|
| Format |
Microplates
|
| Protocol |
Standard curve
|
| Shipping Conditions |
Shipped Ambient
|
| Unit Size |
1 vial
|
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