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AlphaScreen cAMP Detection Kit, 50,000 Assay Points
AlphaScreen cAMP Detection Kit, 50,000 Assay Points
AlphaScreen cAMP
| Feature | Specification |
|---|---|
| Application | セカンドメッセンジャー検出 |
| Sample Volume | 10 µL |
Product variants
Unit Size: 1,000 assay points
Part #:
6760635D
Unit Size: 10,000 assay points
Part #:
6760635M
Unit Size: 50,000 assay points
Part #:
6760635R
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaScreen cAMP Detection Kit, 50,000 Assay Points
AlphaScreen cAMP
AlphaScreen cAMP Detection Kit, 50,000 Assay Points
Product information
Overview
AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.
This kit contains enough reagents to run 1,000 wells using a 25 µL reaction volume. Protocol can be found on the Certificate of Analysis.
This product replaces 6760625R.
Specifications
| Application |
Second Messenger Detection
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaScreen
|
| Detection Modality |
Alpha
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
cAMP
|
| Target Class |
GPCR
|
| Technology |
Alpha
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
50,000 assay points
|
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AlphaScreen cAMP Detection Kit, 50,000 Assay Points
AlphaScreen cAMP Detection Kit, 50,000 Assay Points
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Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit
G protein-coupled receptors (GPCRs) are cell surface transmembrane receptors that activate G-proteins. GPCR activity is often...
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