AlphaLISA Human IL-6 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half time of an ELISA assay

Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130/ IL-6R receptor complex, leading to activation of JAK/STAT, MAPK and PI3K cascades.

AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.