Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-TYK2 (Tyr1054/1055) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-TYK2 (Tyr1054/1055) assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-TYK2 (Tyr1054/1055) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-TYK2 (Tyr1054/1055) assay kit, but can also be ordered separately.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-TYK2 (Tyr1054/1055) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were cultured in 10% FBS containing medium and treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes, washed with HBSS and lysed with Lysis Buffer at a final density of 5 x 106 cells/mL.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
Interferon signaling
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
TYK2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
1 vial
|
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