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Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total TBK1 assay. This control is already provided in the AlphaLISA SureFire Ultra Total TBK1 assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total TBK1 assay. This control is already provided in the AlphaLISA SureFire Ultra Total TBK1 assay kit, but can also be ordered separately.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Total TBK1 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 15 minutes, and lysed in 5 mL 1X SureFire Ultra Lysis Buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
TLR signaling
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
TBK1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
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