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AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
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AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 30 µL |
Product variants
Unit Size: 500 assay points
Part #:
ALSU-PPLK1-A500
Unit Size: 10,000 assay points
Part #:
ALSU-PPLK1-A10K
Unit Size: 50,000 assay points
Part #:
ALSU-PPLK1-A50K
Unit Size: 100 assay points
Part #:
ALSU-PPLK1-A-HV
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
Product information
Overview
Polo-like kinase 1 (PLK1) is a Ser/Thr kinase essential for cell division (mitosis) and as such, a key regulator of the G2/M checkpoint of the cell cycle. PLK1 is activated by phosphorylation of Thr210 by AurA kinase and active PLK1 phosphorylates CDC25. Overexpression of PLK1 is associated with many types of cancers, including lung, breast, liver, and osteosarcoma.
The AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-PLK1 (Thr210) in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
How it works
Phospho-AlphaLISA SureFire Ultra assay principle
The Phospho-AlphaLISA SureFire Ultra assay measures a protein target when phosphorylated at a specific residue.
The assay uses two antibodies which recognize the phospho epitope and a distal epitope on the targeted protein. AlphaLISA assays require two bead types: Acceptor and Donor beads. Acceptor beads are coated with a proprietary CaptSure™ agent to specifically immobilize the assay specific antibody, labeled with a CaptSure tag. Donor beads are coated with streptavidin to capture one of the detection antibodies, which is biotinylated. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, allowing the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the quantity of phosphoprotein present in the sample.
Phospho-AlphaLISA SureFire Ultra two-plate assay protocol
The two-plate protocol involves culturing and treating the cells in a 96-well plate before lysis, then transferring lysates into a 384-well OptiPlate™ plate before the addition of Phospho-AlphaLISA SureFire Ultra detection reagents. This protocol permits the cells viability and confluence to be monitored. In addition, lysates from a single well can be used to measure multiple targets.
Phospho-AlphaLISA SureFire Ultra one-plate assay protocol
Detection of Phosphorylated target protein with AlphaLISA SureFire Ultra reagents can be performed in a single plate used for culturing, treatment, and lysis. No washing steps are required. This HTS designed protocol allows for miniaturization while maintaining AlphaLISA SureFire Ultra quality.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
30 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
PLK1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
Oncology
|
| Unit Size |
100 assay points
|
Video gallery
AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human and Mouse Phospho-PLK1 (Thr210) Detection Kit, 100 Assay Points
Resources
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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Brochure
Species compatibility for HTRF, AlphaLISA SureFire Ultra and Alpha SureFire Ultra Multiplex assays
This document includes detailed tables listing HTRF™, AlphaLISA™ SureFire® Ultra™, and Alpha SureFire® Ultra™ Multiplex assays...
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