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Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-IRF3 (Ser386) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-IRF3 (Ser386) assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-IRF3 (Ser386) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-IRF3 (Ser386) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-IRF3 (Ser386) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A431 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 200 μg/mL poly (I:C) for 2 hours and lysed in 10 mL of 1X SureFire Ultra Lysis Buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
Inflammasome/Pattern Recognition Receptors (PRRs)
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRF3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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