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AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
Product variants
Unit Size: 100 assay points
Part #:
ALSU-PPERK-B-HV
Unit Size: 500 assay points
Part #:
ALSU-PPERK-B500
Unit Size: 10,000 assay points
Part #:
ALSU-PPERK-B10K
Unit Size: 50,000 assay points
Part #:
ALSU-PPERK-B50K
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
Product information
Overview
Protein kinase-like endoplasmic reticulum kinase (PERK) also known as EIF2AK3 is an eIF2α kinase and transmembrane protein located in the endoplasmic reticulum (ER) membrane, activated by ER stress signals. PERK activation during ER stress involves autophosphorylation of its cytoplasmic kinase domain, with phosphorylation at Thr982 serving as a marker for activation. PERK supports tumor growth by reducing oxidative DNA damage and is linked to neurodegenerative diseases and various cancers.
The AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-PERK (Thr982) in cellular lysates, using Alpha Technology. This kit is specifically intended to quantify PERK and does not detect ERK1/2.
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire Ultra kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
How it works
Phospho-AlphaLISA SureFire Ultra assay principle
The Phospho-AlphaLISA SureFire Ultra assay measures a protein target when phosphorylated at a specific residue.
The assay uses two antibodies which recognize the phospho epitope and a distal epitope on the targeted protein. AlphaLISA assays require two bead types: Acceptor and Donor beads. Acceptor beads are coated with a proprietary CaptSure™ agent to specifically immobilize the assay specific antibody, labeled with a CaptSure tag. Donor beads are coated with streptavidin to capture one of the detection antibodies, which is biotinylated. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, allowing the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the quantity of phosphoprotein present in the sample.
The referenced media source is missing and needs to be re-embedded.
Phospho-AlphaLISA SureFire Ultra two-plate assay protocol
The two-plate protocol involves culturing and treating the cells in a 96-well plate before lysis, then transferring lysates into a 384-well OptiPlate™ plate before the addition of Phospho-AlphaLISA SureFire Ultra detection reagents. This protocol permits the cells viability and confluence to be monitored. In addition, lysates from a single well can be used to measure multiple targets.
The referenced media source is missing and needs to be re-embedded.
Phospho-AlphaLISA SureFire Ultra one-plate assay protocol
Detection of Phosphorylated target protein with AlphaLISA SureFire Ultra reagents can be performed in a single plate used for culturing, treatment, and lysis. No washing steps are required. This HTS designed protocol allows for miniaturization while maintaining AlphaLISA SureFire Ultra quality.
The referenced media source is missing and needs to be re-embedded.
Assay validation
Activation of Phospho PERK (Thr982) in Thapsigargin treated cells
SH-SY5Y cells were seeded in a 96-well plate (40,000 cells/well) in complete medium, and incubated overnight at 37°C, 5% CO2. The cells were treated with increasing concentrations of Thapsigargin for 30 minutes.
After treatment, the cells were lysed with 100 µL of Lysis Buffer for 10 minutes at RT with shaking (350 rpm). PERK Phospho (Thr982) and Total levels were evaluated using respective AlphaLISA SureFire Ultra assays. For the detection step, 10 µL of cell lysate (approximately 4,000 cells) was transferred into a 384-well white OptiPlate, followed by 5 µL of Acceptor mix and incubated for 1 hour at RT. Finally, 5 µL of Donor mix was then added to each well and incubated for 1 hour at RT in the dark. The plate was read on an Envision using standard AlphaLISA settings.
As expected, Thapsigargin triggered a dose-dependent increase in the levels of Phospho PERK (Thr982) while Total PERK levels remained unchanged.
Activation of Phospho PERK (Thr982) in Tunicamycin treated cells
SH-SY5Y cells were seeded in a 96-well plate (40,000 cells/well) in complete medium, and incubated overnight at 37°C, 5% CO2. The cells were treated with increasing concentrations of Tunicamycin for 2 hours.
After treatment, the cells were lysed with 50 µL of Lysis Buffer for 10 minutes at RT with shaking (350 rpm). PERK Phospho (Thr982) and Total levels were evaluated using respective AlphaLISA SureFire Ultra assays. For the detection step, 10 µL of cell lysate (approximately 8,000 cells) was transferred into a 384-well white OptiPlate, followed by 5 µL of Acceptor mix and incubated for 1 hour at RT. Finally, 5 µL of Donor mix was then added to each well and incubated for 1 hour at RT in the dark. The plate was read on an Envision using standard AlphaLISA settings.
As expected, Tunicamycin triggered a dose-dependent increase in the levels of Phospho PERK (Thr982) while Total PERK levels remained unchanged.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Host Species |
Human
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
PERK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
100 assay points
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points
Resources
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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog
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Species compatibility for HTRF, AlphaLISA SureFire Ultra and Alpha SureFire Ultra Multiplex assays
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