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AlphaLISA SureFire Ultra Human and Mouse Phospho-NF-κB p65 (Ser536) Biotin Free Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human and Mouse Phospho-NF-κB p65 (Ser536) Biotin Free Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Control Lysate
Extra control lysate for the AlphaLISA™ SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay kit but can also be ordered separately.
Product variant
Unit Size: 1 vial
Part #:
ASBF-PNFKB-A-L
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human and Mouse Phospho-NF-κB p65 (Ser536) Biotin Free Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Control Lysate
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Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Biotin-Free Human and Mouse p-NFκB p65 (Ser536) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing media, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 10 minutes and lysed with 10 mL of Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Biotin-Free
|
| Cellular or Signaling Pathway |
Inflammasome/Pattern Recognition Receptors (PRRs)
|
| Detection Modality |
Alpha
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
NF-κB p65
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
Oncology
|
| Unit Size |
1 vial
|
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