In the rapidly evolving field of molecular biology, the extraction and analysis of small RNA from plasma have become pivotal for biomarker discovery and next-generation sequencing (NGS) applications. Revvity’s application note, “Optimizing the Extraction of Small RNA from Plasma for NGS Applications,” introduces a groundbreaking automated workflow that promises to revolutionize this process.

The Importance of Small RNA

MicroRNAs (miRNAs) are small, non-coding RNA molecules that play a crucial role in regulating protein levels within cells. Their stability in extracellular spaces and tissue-specific expression profiles make them ideal biomarkers for various health conditions. However, extracting miRNA from plasma presents significant challenges due to the low and variable amounts of RNA and the presence of fragmented RNA molecules that can interfere with downstream analysis.

Revvity’s Innovative Solution

Revvity addresses these challenges with a high-throughput, automated method for extracting circulating free RNA from small plasma volumes. This method ensures high yield and purity, making the RNA suitable for small RNA library preparation and massively parallel sequencing.

Key Features of Revvity’s Workflow:

• Consistent and Scalable Extraction: Achieve yields comparable to manual methods with a fast, automated workflow.
• Minimal Hands-On Time: Only 10 minutes of hands-on time required, with on-board lysis and no heating needed.
• Versatile Sample Compatibility: Suitable for plasma, serum, or extracellular vesicles with input volumes of 200 µL or 1 mL.
• Proven Compatibility: Seamlessly integrates with Revvity NEXTFLEX® Small RNA-Seq Kit v4 for various downstream assays.

Revvity’s chemagic™ miRNA 200 H96 Kit, combined with the NEXTFLEX® Small RNA-Seq Kit v4, enables a fully automated, high-throughput workflow for small RNA extraction and sequencing. This innovation opens new possibilities for biomarker discovery and research applications, providing researchers with a reliable and efficient method to unlock the potential of small RNA biomarkers.

For research use only. Not for use in diagnostic procedures.