A streamlined metagenomic workflow for microbiome research from stool

Stool is an established and common sample type for microbiome research thanks to its non-invasive and relatively simple collection. 

Many labs have leveraged stool metagenomics / metagenomes to gain insights into gut health such as in the context of the association of an individual’s gut microbiome in diverse studies encompassing human cancers, neurological disorders including neurodegeneration, as well as systemic diseases such as Type I diabetes or IBD.

As a complex matrix, thorough sample homogenization and effective microbial lysis is a priority for those looking to avoid bias and misrepresentation of community populations. Though various sample homogenization technologies exist, bead mill homogenizers  offer a robust and streamlined solution to help process samples for analytical reproducibility and concerns from cross-contamination.

Access our quick-look flyer for the complete metagenomic workflow and the difference in data that optimized vs non-optimized bead matrices make for WGS library prep, DNA yields, and alpha diversity from our proof-of-concept work using mouse stool.

For research use only. Not for use in diagnostic procedures