A simple, reliable, no-wash assay platform for AAV capsid titer quantification based on HTRF technology

Accurately measuring the AAV concentration in complex biological matrices is critical for the safe and effective manufacturing and clinical utilization of AAV gene therapies. Differences in the AAV capsid of each serotype give rise to tropism, resulting in varying cell/tissue-specific viral transduction efficiencies. This reflects the need for the development of analytical tools that cover multiple AAV serotypes used in the development of human gene therapies.

In this application note you will discover a reliable and efficient method for quantifying AAV titers. The following assay parameters were determined for AAV1, AAV2, AAV3B, AAV5, AAV6, AAV8, and AAV9 serotypes:

• AAV capsid cross-reactivity.
• Intra- and inter-assay precision.
• Assay sensitivity (LOD, LOQ, dynamic range).
• Detection of both empty and full AAV capsids.
• Spike-recovery from samples diluted in buffer, cell culture medium, and cell lysate.

For research use only. Not for use in diagnostic procedures.