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Application Note
RNA extraction from Saccharomyces cerevisiae on the Omni Bead Ruptor Elite bead mill homogenizer
Saccharomyces cerevisiae, yeast, is a commonly used model organisms for studying cellular processes in translational research. Traditional enzymatic methods for extracting yeast RNA and intracellular proteins can be time-consuming and can lead to the denaturation of some proteins. This application note introduces the use of the Omni Bead Ruptor Elite bead mill homogenizer for mechanical disruption of yeast cells, which can quickly and effectively disrupt yeast cell walls for extraction of biological molecules such as RNA. The extraction efficiency and analyte integrity resulting from this method were evaluated.
For research use only. Not for use in diagnostic procedures.
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RNA extraction from Saccharomyces cerevisiae on the Omni Bead Ruptor Elite bead mill homogenizer