pHSense assays and reagents: Guidelines for successful monitoring of cell surface binder internalization

This guide provides everything you need to design, perform, and analyze pHSense™ internalization assays. Learn about the assay principle, experimental workflow, and best practices for assay setup, including cell preparation, reagent handling, and execution protocols.

The guide also covers signal acquisition and data analysis approaches, including FOC, EC₅₀, and kinetic measurements, along with expert recommendations for assay optimization to support robust and reproducible results.

What you'll learn:

• Assay principle and mechanism of action
• Experimental workflow and setup considerations
• Cell preparation and reagent handling best practices
• Step-by-step assay execution protocols
• Signal acquisition and data analysis (FOC, EC50, kinetics)
• Critical parameters and optimization strategies for reproductible results

For research use only. Not for use in diagnostic procedures.