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Application Note

Multidimensional screening platform of GPCR-targeting compounds with HTRF assays

GPCR signaling is inherently multidimensional, and single-endpoint assays may overlook critical pharmacological responses. Discover how combining HTRF™ cAMP, IP-One and GTP/Gi assays provides a comprehensive characterization of receptor activity across Gαs, Gαq and Gαi pathways.

Key takeaways:

Assess GPCR activity across multiple signaling pathways using a unified HTRF workflow.
Differentiate agonist pharmacology through orthogonal functional readouts.
Gain deeper insights into receptor biology to support hit identification and lead optimization.

For research use only. Not for use in diagnostic procedures.

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Multidimensional screening platform of GPCR-targeting compounds with HTRF assays

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