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Application Note
Multi-parametric assessment of EGF treatment effects on signaling pathways, growth and proliferation using AlphaLISA SureFire Ultra and cellular imaging
In this application note, we demonstrate an efficient cell-based workflow for the assessment of EGF treatment effects in a cellular model of human skin cancer.
Treatment effects on several intracellular signaling pathways were examined using Revvity’s homogeneous, no-wash AlphaLISA™ SureFire® Ultra assays.
To determine concurrent time-dependent effects of different EGF concentrations on cellular health and proliferation, ATP concentrations were assessed with ATPlite® 1step luminescence assay and cultures were fluorescently labeled, imaged and analyzed using the Operetta CLS™ high-content analysis system.
For research use only. Not for use in diagnostic procedures.
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Multi-parametric assessment of EGF treatment effects on signaling pathways, growth and proliferation using AlphaLISA SureFire Ultra and cellular imaging