Maximizing efficiency with well-plate tissue lysis coupled with dual DNA and RNA extraction without sample splitting

Biological tissues and complex matrices can be tough-to-lyse and are inherently heterogenous. Genomic and transcriptomic assessment is an important component to multiomic studies that can provide an integrated view into biological processes.

Traditional nucleic acid extraction methods tend to require sample splitting into separate tubes of tissue or cell lysate, one for DNA and one for RNA. Though this may suffice for some lab applications, it may be inefficient from a high-throughput workflow standpoint. Even more so, if labs are working with limited and precious samples such as biospecimens or biopsies for clinical research. Having a unified workflow that enables the simultaneous extraction of DNA and RNA, without sample splitting, may help labs gain integrated insights or more subtle changes in transcriptomic profiles that might have been missed when performing assays, such as bulk sequencing. Coupling this technology with a 96-well plate bead mill homogenizer helps maximize process efficiency.

In this application note

• Learn about the dual extraction protocol, leveraging well-plate homogenization at the front end of extraction.
• Benchmark against your existing bead mills or tissue lysis method with homogenization parameters used for mouse liver, kidneys, brain, heart, and shaved skin with homogenization parameters
• Review data at two sample sizes of tissue (mg) for yield and quality metrics for DNA and RNA, not limited to DNA fragment size, 260/280 purity ratios, and RIN values.

For research use only. Not for use in diagnostic procedures.