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Technical Note
Increasing high-content imaging throughput with cell-line multiplexing
Our technical note describes the multiplexing of 4 different cell lines in a single well using Semarion’s SemaCyte® optically-barcoded microcarriers, the Opera Phenix™ Plus high-content screening system and Harmony™ image analysis software.
Using the cell painting assay and phenotypic profiling, we show that microcarriers from multiplexed wells cluster with the correct cell lines, confirming successful multiplexing.
Key benefits:
- 4x throughput increase - analyze multiple cell lines simultaneously per well
- Reduced reagent costs - fewer wells needed
- Automated workflow - dedicated Harmony software building block streamlines detection
- Verified performance - correct clustering by cell type.
For Research Use Only. Not for use in diagnostic procedures.
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Increasing high-content imaging throughput with cell-line multiplexing