Homogenization of candy or sweets for porcine DNA food testing

Halal and Kosher dietary laws prohibit the use of products containing porcine origin. Pork-based additives, such as collagens used in gelatin or as gelling agents in various processes, are surprisingly common in a variety of food and pharmaceuticals. Bead milling, such as with the Omni Bead Ruptor Elite, offers a high-throughput solution for homogenizing samples for DNA or protein extraction. Methods such as PCR or LC-MS/MS are conductive and crucial for detecting porcine-derived collagen. Bead milling using single-use, sealed tubes are particularly effective for challenging confectionery matrices, and requires no hazardous cleaning solutions; it also offers an alternative that significantly reduces processing time compared to single-sample, manual methods. Herein, we provide recommended parameters for bead mill homogenization of candy.

For research use only. Not for use in diagnostic procedures.