High-throughput mosquito DNA extraction and PCR analysis on the Omni Bead Ruptor 96 bead mill homogenizer

Field sampling of mosquito followed by sample dissociation and DNA purification is a routine process for research that surveys the spread of vector-borne diseases. Researchers require a fast and repeatable sample preparation technique to rapidly dissociate a large number of mosquitoes while maintaining a high degree of vector and internal pathogen lysis for quantification of pathogen load. As the specimen sizes are relatively small and the number of samples is high, bead beating is an ideal method for high-throughput sample disaggregation. Herein, we demonstrate dissociation of Aedes aegypti using the Omni Bead Ruptor 96 bead mill homogenizer for the dissociation of mosquitos in both well-plates and tube format and assessment of the extracted DNA from homogenates by endpoint PCR.

For research use only. Not for use in diagnostic procedures.