High-content imaging of brain-on-chip microfluidic devices using PreciScan intelligent acquisition.

Brain-on-chips (BoC) are innovative tools that simulate complex brain environments, enhancing our understanding of neurodegenerative diseases (NDDs). While a range of BoC platforms have been developed to model diverse NDD pathophysiologies, the various channel architectures of these microfluidic devices present challenges in image acquisition and analysis. High-content imaging protocols must be adaptable to these differing designs.

This technical note, developed in collaboration with ETAP-Lab, demonstrates an automated image acquisition protocol for BoC microfluidic devices. The protocol utilizes the Operetta CLS^™ high-content analysis system with PreciScan intelligent acquisition to effectively image neuronal cells in NETRI microfluidic devices.

We address:

• How to automate image acquisition of microfluidic devices
• How to set up a pre-scan re-scan routine for selected relevant channels within brain-on- chip devices
• How to increase throughput for quantitative microscopy-based screening of brain-on-chip models

For research use only. Not for use in diagnostic procedures.