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Genetic extraction and analysis from weathered 6-year old Bos taurus bones through bead mill homogenization
DNA analysis of skeletal remains is useful in various scientific fields as bones are known to preserve DNA longer than soft tissues. The extraction of genetic material from bones, however, is challenging due to the degradation of DNA over time and under different environmental conditions. We describe uses of a combination of chemical and mechanical methods to extract DNA from aged Bos taurus bones, focusing on overcoming the low yield of genetic recovery and preventing further degradation compared to fresh tissue.
For research use only. Not for use in diagnostic procedures.
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Genetic extraction and analysis from weathered 6-year old Bos taurus bones through bead mill homogenization