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Application Note
Download your 3 application notes on kinase inhibitor selection for kinase binding assays
Challenge kinase binding assays and win with HTRF™ technology
The 3 application notes present a new HTRF kinase binding assay that combines the usage of a fluorescent inhibitor with a tagged kinase enzyme and its corresponding anti-tag-Europium labeled detection reagent.
These 3 application notes will provide you with:
- Validation data for the 3 fluorescent tracers Dasatinib-red, Staurosporine-red and Sunitinib-red
- Guidance for the determination of inhibitor constants: Kd and Ki
- Pharmacological characterization of known inhibitors and correlation with literature data
- Relevant case studies to help you revolutionize your assays
For research use only. Not for use in diagnostic procedures.
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Application note
HTRF kinase binding assays: Dasatinib-Red validation
This note presents a new HTRF
kinase binding assay that combines
the usage of a Dasatinib-Red ligand
with a tagged kinase enzyme and
its corresponding anti-tag-europium
labeled detection reagents.
Application note
HTRF kinase binding assays: Staurosporine-Red validation
This note presents a new
HTRF kinase binding assay
that combines the usage of
a Staurosporine-Red ligand
with a tagged kinase enzyme
and its corresponding
anti-tag-Europium labeled
detection reagents.
Application note
HTRF kinase binding assays: Sunitinib-Red validation
This note presents a new HTRF®
kinase binding assay that combines
the usage of a Sunitinib-Red ligand
with a tagged kinase enzyme and its
corresponding anti-tag- Europium
labeled detection reagents.