Development and validation of customized guide RNA design and efficiency prediction tools for the Pin-point base editing platform

This scientific poster describes the development and validation of customized guide RNA design and efficiency prediction tools for the Pin-point base editing platform.

To facilitate and accelerate the development of applications based on the Pin-point platform, we created a custom tool to design gRNAs to target the gene of interest and to install base conversion, including those that would either introduce premature STOP codons or destroy splice sites to knockout the target gene. In addition, we performed a parallel cell-based screen to analyze the editing activity of two different Pin-point base editor configurations with gRNAs targeting thousands of target sequences.

Pin-point™ base editing reagents are available for research use only and are not for diagnostic use or direct administration into humans or animals. The Pin-point™ base editing platform technology is available for clinical or diagnostic study and commercialization under a commercial license from Revvity.

For research use only. Not for use in diagnostic procedures.