Detecting changes in phosphorylation events during the cell cycle

Lamin A/C, a crucial nuclear protein, undergoes phosphorylation at mitotic sites, controlled by the CDK1-Cyclin B1 complex, impacting cell cycle regulation, morphology, and fate. Dysregulation of phosphorylated Lamin A/C and associated proteins is linked to increased cell growth, genomic instability, and cell senescence in cancer, highlighting a promising target for therapeutic interventions.

AlphaLISA^™ SureFire^® Ultra^™ technology provides a sensitive and efficient approach to monitor cell cycle dynamics and maintain cellular balance, even with low cell numbers. This technical note demonstrates its ability to detect changes in phosphorylated and total cell cycle regulatory proteins within the same cell lysate sample, offering a powerful tool for understanding complex cell biology and aiding drug discovery efforts. Learn more.

For research use only. Not for use in diagnostic procedures.