Combining high-content imaging and DRUG-seq to improve mechanism-of-action profiling

This application note demonstrates how combining cell painting with MERCURIUS™ DRUG-seq transcriptomics delivers a more complete picture of compound activity. By pairing high-content imaging with gene expression profiling, researchers can:

• Resolve subtle mechanistic differences between compounds with similar phenotypes
• Prioritize hits with confidence using orthogonal, high-dimensional data
• Accelerate MoA classification with AI-ready datasets that integrate morphological and transcriptomic signatures

What you’ll learn:

• How Cell Painting and DRUG-seq complement each other to uncover mutation-selective KRAS pathway responses
• Strategies for distinguishing bromodomain inhibitors using transcriptional precision
• Best practices for integrating phenotypic and transcriptomic data into your screening workflow

For research use only. Not for use in diagnostic procedures.