Characterization of CD34+ hematopoietic stem cells from human umbilical cord blood with Cellaca PLX image cytometer

Hematopoietic stem cells (HSCs), identified by the surface marker CD34, are rare, multipotent cells derived from sources like umbilical cord blood (UCB) and are critical to research in genome editing, regenerative medicine, and immune modulation. Ensuring the quality of CD34+ HSC products requires robust characterization of key critical quality attributes (CQAs), including cell count, viability, purity, and potency. This poster presents a streamlined workflow using Revvity's Cellaca™ PLX image cytometer to characterize UCB CD34+ HSCs through a dual-fluorescence AO/PI viability assay and a four-color multiplexed surface marker assay, offering a user-friendly, visually intuitive alternative to conventional flow cytometry.

Key learnings:

• Cellaca PLX image cytometer delivers comparable results to flow cytometry Across three vials of post-thaw UCB CD34+ HSCs, the Cellaca PLX produced highly comparable results to a conventional flow cytometer across all major CQAs, including CD34+ population, viability, and viable cell count.
• A single, streamlined workflow covers multiple CQAs The Cellaca PLX workflow integrates AO/PI viability assessment and a multiplexed four-color surface marker assay in one platform, enabling automated analysis of CD34+ population, viability, purity, and viable cell count, all critical for in-house quality control before downstream applications.
• CD34+ HSCs retain functional potency post-thaw A colony-forming unit (CFU) assay confirmed that the UCB CD34+ HSCs maintained their differentiation potential after thawing, successfully forming multiple colony types.

For research use only. Not for use in diagnostic procedures.