Characterising ADC activity beyond cytotoxicity: A cell-based screening workflow

Evaluating antibody-drug conjugates (ADCs) using viability alone can miss important differences in receptor internalisation, payload activity and bystander effects. An integrated cell-based workflow helps connect these mechanisms to functional outcomes and supports more informed ADC development decisions.

Read this scientific poster from our preclinical services team shows how the workflow helps:

• Profile surface receptos: Classify cell models by HER2 expression to support selection of relevant models for ADC evaluation.
• - Measure receptor internalization: Compare ADC processing kinetics and identify a practical 6 to 24-hour window for assessing HER2-driven internalization.
• Compare functional activity: Evaluate ADC potency and efficacy in both 2D and 3D models, revealing differences that may be missed in a single culture format.
• Distinguish payload and bystander effects: Assess payload sensitivity independently of HER2 expression and investigate differences in bystander killing.
• Connect molecular and functional readouts to build a more complete picture of ADC behaviour and support candidate prioritization.

Download the poster now.

For research use only. Not for use in diagnostic procedures.