Bead mill-based plant protein extraction efficiency is not dependent upon starting sample quantity

Bead mill homogenizers enable rapid and efficient lysis of biological materials, including plants via high-impact forces generated from beads in an enclosed tube to dissociate samples. We explore the efficiency of vertical intra-tube bead milling motions of 7mL and 30mL tube and spiral grinding motions of a 50mL tube using the Omni Bead Ruptor Elite unit. Results indicate protein extraction yields were consistent run-to-run, in different tube sizes, and that full homogenization was achieved in less than a minute for all cases using the same homogenization settings within a given sample type. Thus, having one unit to support varying sample tube sizes allows bead milling to be leveraged for rapid and efficient sample homogenization across various sample input sizes/volumes for protein applications.

For research use only. Not for use in diagnostic procedures.