Automated microRNA purification with chemagic 360 instrument

Liquid biopsies have shown to be important sources for markers to help detect cancer in its initial stages. Markers such as circulating cell-free DNA and RNA (cfDNA and cfRNA), circulating tumor cells (CTCs) and exosomes have shown to be useful. Analysis of microRNA (miRNA) in plasma samples opens a wide range of interesting information for many research applications. They play roles in both homeostasis in healthy individuals and diseases such as cancer, diabetes, in addition to cardiovascular and kidney pathologies. The isolation of miRNA represents a significant challenge due to low concentrations, small fragment sizes of about 19-22 nucleotides (nt) and furthermore, miRNAs are often bound in protein-complexes contaminating proteins or inhibitors.

In this application note, we demonstrate the efficient automated isolation of high-quality miRNA from plasma using our chemagic miRNA 200 Kit H96 and chemagic™ miRNA 1k Kit H24 on the chemagic 360 instrument.

For research use only. Not for use in diagnostic procedures.