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Technical Note
AlphaLISA ligand binding assays for measurement of human Immunoglobulin G subclasses in animal sera
This technical note describes AlphaLISA™ ligand binding assays for measuring human IgG subclasses (IgG1 and IgG4) in animal sera for pharmacokinetic applications. The assay relies on a homogeneous, no-wash format using donor and acceptor beads to quantify antibody levels across multiple species.
Key highlights
- AlphaLISA assay for detecting human IgG1 and IgG4 in monkey, mouse, and rat sera.
- Homogeneous, no-wash workflow based on donor and acceptor bead proximity for streamlined immunodetection.
- Demonstrated performance with consistent spike recovery and dilutional linearity across serum matrices.
For research use only. Not for use in diagnostic procedures.
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AlphaLISA ligand binding assays for measurement of human Immunoglobulin G subclasses in animal sera