Evaluating pharmaceutical compound efficacy and safety in regulating cell behavior can involve the study of multiple signal transduction pathways and measuring more than one cellular target can provide greater confidence of positive compound hits on the cellular target of interest. Protein phosphorylation has been identified as a useful readout of cellular activation or inhibition, and these pathways are commonly targeted for therapeutic modulation of disease.

Numerous assay technologies are available to look at protein phosphorylation for drug development, including the AlphaLISA^™ SureFire^® Ultra which provides a highly sensitive, fully homogenous option for the analysis of signaling pathways. This application note details the implementation of multiplexing with the AlphaLISA SureFire Ultra Multiplex assay, which offers a homogenous and high throughput approach to multiplexing.

For research use only. Not for use in diagnostic procedures.