Automate the T-SPOT.TB Workflow

Automation solutions for every lab size

The T-SPOT.TB test can now be automated allowing for increased workflow efficiency and optimized hands-on time for latent tuberculosis testing. With our range of solutions, you can select the most cost-effective combination for your specific needs.

The T-SPOT.TB test enables accurate TB testing1 and when paired with our automation solutions you get efficient laboratory workflows without compromising on clinical performance.2 This powerful combination not only simplifies T-SPOT.TB testing but also provides confidence in the result.
 

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Choose your perfect level of automation

Only one single primary blood tube is needed throughout the T-SPOT.TB workflow

Extraction to analysis: Metagenomics workflows: 16 sequencing, WGS, and metatranscriptomics
Extraction to analysis: Metagenomics workflows: 16 sequencing, WGS, and metatranscriptomics

Cell isolation - A range of options to match your workflow

Isolate & wash PBMCs using +ve selection

Cell isolation
Auto-Pure 10B

Auto-Pure 10B

  • Up to 10 samples to be processed at one time (~45 minute processing time)
  • Simple to use and maintain with an easy-to-use touch screen
  • Compact design minimises bench space usage
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Auto-Pure 20B

Auto-Pure 20B

  • Up to 20 samples to be processed at one time (~45 minute processing time)
  • Simple to use and maintain with an easy-to-use touch screen
  • Compact design minimises bench space usage
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Auto-Pure 24

Auto-Pure 24

  • Up to 24 samples to be processed at one time (~45 minute processing time)
  • Plate format allows integration into fully automated liquid handler workflow
  • Easy to use with minimal maintenance
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Auto-Pure 2400

Auto-Pure 2400

  • Combines cell isolation and plate preparation capabilities for Day 1 of the T-SPOT.TB test
  • 24 samples per run in 3.5 hours with a single mid-run user interaction
  • Less than 10 mins hands-on time once the Auto-Pure 2400 workflow has commenced*
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Cell counting

Enumerate isolated PBMCs

Cell counting
Cellaca MX

Cellaca MX

  • Fast run time: ~3 mins per plate
  • Easy to use
  • Minimal maintenance
Learn more

Plate preparation

Normalise & plate PBMCs

Plate preparation
Auto-Pure 2400

Auto-Pure 2400

  • Combines cell isolation and plate preparation capabilities for Day 1 of the T-SPOT.TB test
  • 24 samples per run in 3.5 hours with a single mid-run user interaction
  • Less than 10 mins hands-on time once the Auto-Pure 2400 workflow has commenced
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Janus G3

Janus G3

  • Customizable plate preparation workstation
  • Flexibility in throughput
  • Dynamic volume range

Revvity offer a range of plate preparation platforms:

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Plate development

Wash and add substrate & conjugate

Plate development
Janus G3

Janus G3

  • Customizable plate preparation workstation
  • Flexibility in throughput
  • Dynamic volume range

Revvity offer a range of plate preparation platforms:

Learn more

Review T-Cell produced interferon gamma spots

Microwell Imager

Spot counting
Euroimmun Microwell Imager

Microwell Imager

  • Plate imaging system with software to support users with interpretation of results
  • Directly visualizes the T cell produced interferon-y spots
  • Less than 2 mins processing time for 24 samples with compact footprint
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* Pipetting steps can be easily performed manually or automated for a more efficient workflow

** Each result should be individually validated before reporting

NEW: Auto-Pure 2400 - CE IVD and FDA approved

Designed for testing up to 24 samples per run, the Auto-Pure 2400 is an automated liquid handling platform with integrated cell isolation technology.

The Auto-Pure 2400 can perform day one T-SPOT.TB workflows in under 3.5 hours with a single mid-run user interaction.

  • Efficient workflow, more time for you.
  • Easy to use, easy to get right.
  • Access high-quality testing, be confident in the result.
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Less than 10 mins hands-on time once the Auto-Pure 2400 workflow has commenced*

Automate the T-SPOT.TB Workflow

Benefits at a glance

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Only one single primary tube needed**

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Room temperature (18° – 25°C) sample storage1

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54-hour sample stability2

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Low indeterminate results, few repeat tests3

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Maintains performance in the immunosuppressed4,5

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High sensitivity and specificity providing accurate results1

Case study

Oxford Diagnostic Laboratories (ODL) is transforming the landscape of TB testing with the introduction of the new Auto-Pure 2400 with the T-SPOT.TB test.

Download case study
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Hear from our customers

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customer elliot derby

“We have been convinced users of the T-SPOT.TB test for many years and are very satisfied with the performance and results, especially in patients with immunosuppression.
The new automation solutions not only enable more flexible sample logistics and laboratory organisation but also lead to a higher cell yield with consistently high cell quality.”

Dr Martin Obermeier
Director and Head of Laboratory
MIB -Medical and Infectious Diseases Centre Berlin

"The implementation of the Auto-Pure 2400 with the T-SPOT.TB test has already yielded some exciting results, with zero occurrences of low PBMC rates (insufficient cells) and 100% result concordance across testing. This data includes samples received from immunosuppressed individuals"


Elliot Derby
Technical Scientist
Oxford Diagnostic Laboratories (Trading division of Oxford Immunotec Limited, a Revvity company)

Expert demo previews

Meet our T-SPOT automation specialists as they give you a sneak peek into our cutting-edge solutions

Automate the T-SPOT.TB Workflow demo preview 1

Watch James demonstrate the Auto-Pure 2400 in action with the T-SPOT.TB 
test. This lab-based teaser shows how our automation solution
streamlines the testing process, improving efficiency
and consistency in your TB testing workflow

Automate the T-SPOT.TB Workflow demo preview 2

Join Yasmin at ESCMID as she walks through the complete T-SPOT.TB workflow on the Auto-Pure 2400 platform. See first-hand how this integrated solution transforms laboratory operations from sample
preparation to results analysis

Explore our T-SPOT related solutions

T cell immunology

T-SPOT technology

Learn more on our T-SPOT product portfolio and how this technology plays a vital role in various clinical applications, aiding in the detection of infections and supporting the monitoring of immune responses.

Learn more
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Tuberculosis management

Learn about TB, its impact, and the importance of accurately detecting latent infections using advanced IGRA tests like the T-SPOT.TB test, for effective TB management.

Learn more
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Investigator Led Studies at Revvity

We are committed to improving the care of patients with immune-regulated conditions by providing funding and other forms of support to qualified investigators who share our commitment and work within our areas of interest.

Learn more
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Products & services
Resource library

Reference

  1. Oxford Immunotec. T-SPOT.TB Package Insert PI-TB-IVD-UK-V7. Abingdon, UK. September 2024. Oxford Immunotec. T-SPOT.TB Package Insert PI-TB-US-0001 v14. Abingdon, UK. November 2023
  2. T-Cell Select Package Insert PI-TS-IVD-UK-V9 Abingdon, UK. September 2024. T-Cell Select Package Insert PI-TS-IVD-US v6. Abingdon, UK. March 2025.
  3. Rego K, et al. Utility of the T-SPOT®.TB test’s borderline category to increase test resolution for results around the cut-off point. Tuberculosis. 2018;108:178
  4. Wong SH, Gao Q, Tsoi KK, Wu WK, Tam LS, Lee N, Chan FK, Wu JC, Sung JJ, Ng SC. Effect of immunosuppressive therapy on interferon γ release assay for latent tuberculosis screening in patients with autoimmune diseases: a systematic review and meta-analysis. Thorax. 2016 Jan
  5. Clark SA, Martin SL, Pozniak A, et al. Tuberculosis antigen-specific immune responses can be detected using enzyme-linked immunospot technology in human immunodeficiency virus (HIV)-1 patients with advanced disease. Clin Exp Immunol. 2007;150(2):238-244.

*Mid run user intervention with Auto-Pure 2400 workflow with Cellaca MX
**In immunosuppressed individuals two tubes may be requested

T-SPOT.TB, Auto-Pure 2400, 24, 20B and 10B: For in vitro diagnostic use.

Cellaca MX & Microwell Imager: For Research Use Only. Not for use in diagnostic procedures.

Products may not be licensed in accordance with the laws in all countries. Please check with your local representative for availability.

Copyright ©2025, Revvity, Inc. All rights reserved. Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.

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