Automated normalization of small RNA libraries with AutoNorm™ technology.

Variability in microRNA abundance across tissues can make small RNA library amplification unpredictable, leading to inconsistent yields and uneven sequencing coverage.

Revvity's new application note demonstrates how the iconPCR™ system (n6), featuring AutoNorm™ technology, brings adaptive control to this critical step. Instead of applying a fixed number of PCR cycles, the system actively monitors amplification in real time and stops each reaction at a defined fluorescence threshold. The result: balanced library output across diverse RNA inputs without manual adjustment.

When combined with the NEXTFLEX™ Small RNA-Seq Kit v4, AutoNorm™ technology enables:

• Reduced variability across sample types and input amounts
• Optimized PCR cycling, preventing over- or under-amplification
• Improved reproducibility and simplified pooling for sequencing

Explore how this integrated solution streamlines small RNA-Seq workflows — improving consistency while saving time.