Measure influenza viral titration with mKate2 fluorescent protein reporter in 96-well plate
- MDCK host cells were seeded in a 96-well microplate and allowed to adhere overnight
- A titration of 5-folds of mKate2-influenza viral particles were prepared and added to the host cells
- The samples were allowed to incubate for 24 hours and then imaged and analyzed with the Celigo™ image cytometer to count the number of infected cells
- The Celigo software was used to identify the total MDCK cells in brightfield images, and then using the fluorescent gating function to determine the percent infection rate
Brightfield and fluorescent images of the individual infected cells in the 96-well. The Celigo software gating shows the counting results of mKate2 positive infected cells.
Utilizing the gating functions in the Celigo, the percentage mKate2 positive cells can be measured to generate an infectivity curve
For research use only. Not for use in diagnostic procedures.